GPx3 knockdown inhibits the proliferation and DNA synthesis and enhances the early apoptosis of human spermatogonial stem cells via mediating CXCL10 and cyclin B1

• DOI: 10.3389/fcell.2023.1213684
• Publication Date: 2023-07-07T14:46:36Z
• Authors: Si Wu; Zixin Cheng; Ye Peng; Ying Cao; Zuping He
• Keywords: Cyclin E1

Spermatogenesis is regulated by genetic and epigenetic factors. However, the genes signaling pathways mediating human spermatogenesis remain largely unknown. Here, we have for first time explored expression, function, mechanism of glutathione peroxidase 3 (GPx3) in controlling proliferation apoptosis spermatogonial stem cells (SSCs). We found that GPx3 was expressed SSCs. Notably, revealed knockdown resulted decrease proliferation, DNA synthesis, cyclin B1 level SSC lines, which possessed phenotypic features primary Flow cytometry TUNEL assays showed silencing led to enhancement early line. RNA sequencing utilized identify CXCL10 as a target SSCs, notably, both double immunostaining co-immunoprecipitation (co-IP) demonstrated there an association between these cells. CXCL10-shRNA reduction synthesis line increase Taken together, results implicate regulates via B1. This study, thus, offers novel insight into molecular regulating fate determinations SSCs spermatogenesis.