Cellular and transcriptomic changes by the supplementation of aged rat serum in human pluripotent stem cell-derived myogenic progenitors

• DOI: 10.3389/fcell.2024.1481491
• Publication Date: 2024-10-17T20:48:01Z
• Authors: Sin-Ruow Tey; Ryan S. Anderson; Clara H. Yu; Samantha Robertson; Heidi Kletzien; Nadine P. Connor; Kaori Tanaka; Yasuyuki Ohkawa; Masatoshi Suzuki
• Keywords: Progenitor

Introduction The changing composition of non-cell autonomous circulating factors in blood as humans age is believed to play a role muscle mass and strength loss. mechanisms through which these act age-related skeletal changes not fully understood. In this study, we used human myogenic progenitors derived from pluripotent stem cells study roles during the process differentiation. Methods Myogenic embryonic (ESCs) induced (iPSCs) were supplemented with serum samples aged or young Fischer 344 × Brown Norway F1-hybrid rats. effect supplementation on progenitor proliferation, myotube formation capacity, differentiation, early transcriptomic profiles analyzed. Results We found that rat significantly reduced cell proliferation increased death both ESC- iPSC-derived progenitors. Next, inhibited maturation terminal differentiation myocytes when compared treated adult serum. Lastly, identified gene expression affected following culture. Discussion Together, caused cellular current data our vitro model possibly simulate contributions processes muscle.