GC–MS profiling of Bacillus spp. metabolites with an in vitro biological activity assessment and computational analysis of their impact on epithelial glioblastoma cancer genes

• DOI: 10.3389/fchem.2023.1287599
• Publication Date: 2023-12-05T07:53:48Z
• Authors: Muhammad Naveed; Huda Ishfaq; Shafique Ur Rehman; Aneela Javed; Muhammad Waseem; Syeda Izma Makhdoom; Tariq Aziz; Metab Alharbi; Abdulrahman Alsha...; Abdullah F. Alasmari
• Keywords: Chemistry; 0303 health sciences; 03 medical and health sciences; PCR; epithelial glioblastoma cancer; Bacillus spp.; biological potential; QD1-999; bacterial metabolites

Background: Bacterial metabolites play a crucial role in human health and have proven effective treating various diseases. In this study, the 16S rRNA method streaking were employed to isolate molecularly identify bacterial strain, with goal of characterizing bioactive volatile extracted using nonpolar polar solvents. Methods: Gas chromatography-mass spectrometry (GC-MS) analysis was conducted 29 compounds metabolites, including key associated Bacillus spp. The main identified included 2-propanone, 4,4-ethylenedioxy-1-pentylamine, 1,2-benzenedicarboxylic acid, 1,1-butoxy-1-isobutoxy-butane, 3,3-ethoxycarbonyl-5-hydroxytetrahydropyran-2-one. Results: literature indicates diverse biological pharmacological applications these compounds. Different concentrations from species tested for activities, revealing significant inhibitory effects on anti-diabetic activity (84.66%), anti-inflammatory (99%), antioxidant (99.8%), anti-hemolytic (90%). Disc diffusion testing also demonstrated noteworthy effect against strains. Conclusion:In silico screening revealed that acid exhibited anticancer promising drug-designing properties epithelial glioblastoma cancer genes. study highlights potential as valuable target drug research, emphasizing significance production antibacterial agents.