Modulation of Rumen Microbes Through Extracellular Vesicle Released by the Rumen Fluke Calicophoron daubneyi

• DOI: 10.3389/fcimb.2021.661830
• Publication Date: 2021-04-20T06:28:43Z
• Authors: Nathan R. Allen; Aspen R. Taylor-Mew; Toby J. Wilkinson; Sharon Huws; Helen Phillips; Russell M. Morphew; Peter M. Brophy
• Keywords: Proteomics; 0301 basic medicine; rumen microbiome; Rumen; Calicophoron daubneyi; Cattle Diseases; Microbiology; QR1-502; Extracellular Vesicles; 03 medical and health sciences; proteomics; Cellular and Infection Microbiology; Animals; Cattle; extracellular vesicle; Trematoda; mass spectrometry

Parasite derived extracellular vesicles (EVs) have been proposed to play key roles in the establishment and maintenance of infection. Calicophoron daubneyi is a newly emerging parasite livestock with many aspects its underpinning biology yet be resolved. This research first in-depth investigation EVs released by adult C. daubneyi. were successfully isolated using both differential centrifugation size exclusion chromatography (SEC), morphologically characterized though transmission electron microscopy (TEM). EV protein components GeLC approach allowing elucidation comprehensive proteomic profiles for their soluble cargo surface membrane bound proteins yielding total 378 identified. Notably, contained Sigma-class GST cathepsin L B proteases, which previously described immune modulation successful parasitic flatworm infections. SEC purified observed modulate rumen bacterial populations likely increasing microbial species diversity via antimicrobial activity. data indicates from role within through regulation offering new routes control fluke infection develop molecular strategies improve efficiency.