Murine Susceptibility to Leishmania amazonensis Infection Is Influenced by Arginase-1 and Macrophages at the Lesion Site

• DOI: 10.3389/fcimb.2021.687633
• Publication Date: 2021-10-01T15:05:59Z
• Authors: Fernanda Tomiotto...; Milena Menegazzo ...; Taylon Felipe Silva; Bruna Taciane da ...; Manoela Daiele Go...; Virginia Márcia C...; Ana Carolina Jaco...; Mariana Barbosa D...; Larissa Staurengo...; Waldiceu Aparecid...; Idessania Nazaret...; Carolina Panis; Ivete Conchon-Costa; Juliano Bordignon; Wander Rogério Pa...
• Keywords: Adoptive Cell Transfer; Leishmania mexicana

Cutaneous leishmaniasis is a zoonotic infectious disease broadly distributed worldwide, causing range of diseases with clinical outcomes ranging from self-healing infections to chronic disfiguring disease. The effective immune response this infection yet be more comprehensively understood and fundamental for developing drugs vaccines. Thus, we used experimental models susceptibility (BALB/c) partial resistance (C57BL/6) Leishmania amazonensis investigate the local profile mediators involved in development cutaneous leishmaniasis. We found worse outcome BALB/c mice than C57BL/6 mice, almost 15 times higher parasitic load, ulcerated lesion formation, levels IL-6 infected paws. In contrast, presented IFN-γ superoxide anion (•O2-) after 11 weeks no ulcerations. A peak macrophages appeared 24 h both studied strains, followed by another increase 240 h, detected only mice. Regarding M1 M2 macrophage phenotype markers [iNOS, MHC-II, CD206, arginase-1 (Arg-1)], pronounced Arg-1 infection, whereas showed an initial predomination profiles, predominance, coinciding second infiltration, infection. Greater deposition type III collagen resolution was also observed adoptive transfer at 11th week reduction edema number parasites site, addition lower Arg-1. have against L. amazonensis, based on balance between inflammation tissue repair, while excessive production late worst evolution seems influenced recruitment related macrophages, since resulted better outcomes,