Pathogen Metagenomics Reveals Distinct Lung Microbiota Signatures Between Bacteriologically Confirmed and Negative Tuberculosis Patients

• DOI: 10.3389/fcimb.2021.708827
• Publication Date: 2021-09-13T16:11:00Z
• Authors: Li Ding; Yanmin Liu; Xiaorong Wu; Minhao Wu; Xiaoqing Luo; Hui Ouyang; Jinyu Xia; Xi Liu; Tao Ding
• Keywords: 0301 basic medicine; bacteriological confirmation; Staphylococcus aureus; bronchoalveolar lavage fluid; Microbiota; pathogen metagenomics sequence; lung microbiota; Mycobacterium tuberculosis; Microbiology; QR1-502; 3. Good health; Kluyveromyces; 03 medical and health sciences; Cellular and Infection Microbiology; tuberculosis; distinct tuberculosis lung microbiota signatures; Ascomycota; Humans; Tuberculosis; Metagenomics; Bronchoalveolar Lavage Fluid; Haemophilus parainfluenzae; Lung

Understanding the dynamics of lung microbiota in tuberculosis patients, especially those who cannot be confirmed bacteriologically clinical practice, is imperative for accurate diagnosis and effective treatment. This study aims to characterize distinct microbial features between negative patients understand influence on patients. We collected specimens bronchoalveolar lavage fluid from 123 Samples were subjected metagenomic next-generation sequencing reveal signatures. By combining conventional bacterial detection sequencing, 101/123 (82%) confirmed. In addition Mycobacterium , Staphylococcus aureus Kluyveromyces lactis Pyricularia pennisetigena also enriched bacteriological confirmation group. contrast, Haemophilus parainfluenzae was Besides, interaction exhibits a different state correlated with characteristics such as albumin chest cavities. Our comprehensively demonstrates correlation unique suggesting importance studying pulmonary microbiome disease providing new insights future precision