An Extracellular Redox Signal Triggers Calcium Release and Impacts the Asexual Development of Toxoplasma gondii

• DOI: 10.3389/fcimb.2021.728425
• Publication Date: 2021-08-10T05:49:04Z
• Authors: Eduardo Alves; Henry J. Benns; Lilian Magnus; Caia Dominicus; Tamás Dobai; Joshua Blight; Ceire J. Wincott; Matthew A. Child
• Keywords: Glutaredoxin; Intracellular parasite

The ability of an organism to sense and respond environmental redox fluctuations relies on a signaling network that is incompletely understood in apicomplexan parasites such as Toxoplasma gondii . impact changes upon the development this intracellular parasite not known. Here, we provide revised collection 58 genes containing domains related canonical antioxidant function, with their encoded proteins widely dispersed throughout different cellular compartments. We demonstrate addition exogenous H 2 O human fibroblasts infected T. triggers Ca 2+ flux cytosol can induce egress. In line existing models, egress triggered by reliant both Calcium-Dependent Protein Kinase 3 diacylglycerol kinases. Finally, show overexpression glutaredoxin-roGFP2 sensor fusion protein parasitophorous vacuole severely impacts replication. These data highlight rich exists , evidencing link between extracellular culminate Our findings also indicate potential environment contributes normal growth. Combined, our important role unexplored regulator biology.