Transcriptional Activity of Predominant Streptococcus Species at Multiple Oral Sites Associate With Periodontal Status

• DOI: 10.3389/fcimb.2021.752664
• Publication Date: 2021-09-21T04:30:37Z
• Authors: Daniel Belstrøm; Florentin Constan...; Merete Markvart; Martin Sikora; Christiane Elisab...; Michael Givskov
• Keywords: Streptococcus salivarius; Dental plaque; Streptococcus sanguinis; Streptococcus gordonii; Oral Microbiome

Background Streptococcus species are predominant members of the oral microbiota in both health and diseased conditions. The purpose present study was to explore if different ecological characteristics, such as oxygen availability presence periodontitis, associates with transcriptional activity genus Streptococcus. We tested hypothesis that genetically closely related express activities samples collected from environments critically conditions determined by site inflammatory status. Methods Metagenomic metatranscriptomic data retrieved 66 samples, subgingival plaque (n=22), tongue scrapings (n=22) stimulated saliva patients periodontitis (n=11) orally healthy individuals (n=11). Species-specific computed Log2(RNA/DNA), compared between multiple sites same individual, Results were identified a site-specific colonization pattern plaque. A total 11, 4 2 pathways expressed S. parasanguinis, infantis salivarius , respectively, recorded significantly higher than biofilm individuals. In addition, 18 pathways, including involved synthesis peptidoglycan, amino acid biosynthesis, glycolysis purine nucleotide biosynthesis parasanguinis 3 less periodontitis. Conclusion Data demonstrates association microbiota. particular, being glycolysis, be associated and/or inflammation