Mining Anti-Inflammation Molecules From Nippostrongylus brasiliensis-Derived Products Through the Metabolomics Approach

• DOI: 10.3389/fcimb.2021.781132
• Publication Date: 2021-11-11T07:42:26Z
• Authors: Yuying Chen; Mingming Zhang; Xin Ding; Yougui Yang; Yujia Chen; Qiang Zhang; Yinwen Fan; Yang Dai; Junhong Wang
• Keywords: Nippostrongylus brasiliensis

Hookworm is one type of soil-transmitted helminth, which could exert an anti-inflammatory effect in human or animal host, provides a beneficial possibility for the discovery inflammatory-related disease interventions. The identification hookworm-derived molecules urgently needed future translational research. emergence metabolomics has become powerful approach to comprehensively characterize metabolic alterations recent times. Herein, excretory and secretory products (ESPs) were collected from cultured adult worm, while small intestinal contents obtained Nippostrongylus brasiliensis ( N. , Nb)-infected mice. Through ultra-high-performance liquid chromatography coupled with mass spectrometry (UHPLC-MS) platform, analysis was used explore molecules. Out 45 differential metabolites that discovered ESPs, 10 them showed potential properties, be subclassed into amino acids, furanocoumarins, linear diarylheptanoids, gamma butyrolactones, alpha-keto acids. In terms derived -infected mice, 14 out 301 demonstrate effects, possible subclassification benzylisoquinolines, quaternary ammonium salts, pyrimidines, pregnane steroids, purines, biphenyls, glycerophosphocholines. Furthermore, nine appeared both ESPs infected contents, wherein four proven show anti-inflammation namely, L-glutamine, glutamine (Gln), pyruvate, alanine-Gln (Ala-Gln). summary, we have provided method parasite-derived properties present study. This array provide clues evaluation study these