Rapid Visual Detection of Hepatitis C Virus Using Reverse Transcription Recombinase-Aided Amplification–Lateral Flow Dipstick

• DOI: 10.3389/fcimb.2022.816238
• Publication Date: 2022-02-17T10:47:20Z
• Authors: Haili Wang; Yuhang Zhang; Jingming Zhou; Ming Li; Yumei Chen; Yankai Liu; Hongliang Liu; Peiyang Ding; Chao Liang; Xifang Zhu; Ying Zhang; Cheng Xin; Gaiping Zhang; Aiping Wang
• Keywords: Recombinase Polymerase Amplification

Hepatitis C virus (HCV) infection is a global public health threat. Reaching the World Health Organization’s objective for eliminating viral hepatitis by 2030 will require precise disease diagnosis. While immunoassays and qPCR play significant role in detecting HCV, rapid accurate point-of-care testing important pathogen identification. This study establishes reverse transcription recombinase-aided amplification–lateral flow dipstick (RT-RAA-LFD) assay to detect HCV. The intact workflow was completed within 30 min, detection limit synthesized C/E1 plasmid gene-containing 10 copies/μl. In addition, test showed good specificity, with no cross-reactivity observed A virus, B HIV, syphilis, human papillomavirus virus. Using extracted RNAs from 46 anti-HCV antibody-positive samples, RT-RAA-LFD 100% positive negative concordance rates qPCR. summary, established this suitable clinical of HCV at community level remote areas.