Microbiome and metabolome associated with white spot lesions in patients treated with clear aligners

• DOI: 10.3389/fcimb.2023.1119616
• Publication Date: 2023-04-04T05:54:49Z
• Authors: Zhixin Song; Shishu Fang; Tao Guo; Yi Wen; Qian Liu; Zuolin Jin
• Keywords: Metabolome

White spot lesions (WSLs) have long been a noteworthy complication during orthodontic treatment. Recently, an increasing number of orthodontists found that adolescents undergoing treatment with clear aligners are at higher risk developing WSLs. The oral microbiota and metabolites considered the etiologic regulatory factors WSLs, but specific impact on is unknown. This study investigated differences in salivary microbiome metabolome between without WSLs treated aligners. Fifty-five (aged 11-18) Invisalign appliances, 27 28 were included. Saliva samples analyzed using 16S rRNA gene sequencing ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS); data further integrated for Spearman correlation analysis. relative abundances 14 taxa, including Actinobacteria , Actinomycetales Rothia Micrococcaceae Subdoligranulum Capnocytophaga Azospira Olsenella Lachnoanaerobaculum Abiotrophia significantly WSL group than control group. Metabolomic analysis identified potential biomarkers, most amino acids, proline glycine. implicated 6 metabolic pathways, alanine, aspartate glutamate metabolism; glycine, serine threonine aminoacyl-tRNA biosynthesis. There was microbial metabolomic datasets, reflecting activity flora. A concordant increase levels some acids had predictive value development. In summary, when undergo long-term aligner therapy poor hygiene habits, can disrupt balance microecosystem lead to dysbiosis, thereby Our findings might contribute understanding pathogenesis provide candidate biomarkers diagnosis associated