Diagnostic accuracy and microbial profiles of tuberculous pleurisy: a comparative study of metagenomic next generation sequencing and GeneXpert Mycobacterium tuberculosis

• DOI: 10.3389/fcimb.2023.1243441
• Publication Date: 2023-11-27T06:05:31Z
• Authors: Fengxiang Huang; Haoran Wang; Ruiping Qiao; Qiang Peng; Chang Zhao; Lijun Miao
• Keywords: GeneXpert MTB/RIF; Pleurisy

Introduction There is a clinical challenge in diagnosing tuberculous pleurisy accurately and promptly, highlighting the urgent need for rapid sensitive diagnostic method. This study aimed to evaluate accuracy of metagenomic next-generation sequencing (mNGS) GeneXpert Mycobacterium tuberculosis (MTB) identifying analyzing microbial profiles both non-tuberculous pleural effusions. Methods The enrolled 31 patients with suspected pleurisy, which 15 were confirmed have subsequently allocated group (TP group), while remaining 16 individuals assigned (NTP group). mNGS MTB performed on effusion samples, tests was compared. We employed established formulas compute crucial indicators, including sensitivity, specificity, missed diagnosis rate, misdiagnosed positive predictive value (PPV), negative (NPV). Results results showed that had high specificity (100%) detecting along comparable sensitivity (46.67% 40.0% MTB). Further analysis combined efficacy test 66.67% 100%. revealed detected 7 out effusions, effusions associated diverse range genera species. most frequently at genus level NTP Microbacterium spp. (6/16), Prevotella (5/16), Campylobacter (5/16). Discussion These findings suggest are useful tools can provide valuable insights into