Etiology of lower respiratory tract in pneumonia based on metagenomic next-generation sequencing: a retrospective study

• DOI: 10.3389/fcimb.2023.1291980
• Publication Date: 2024-01-09T04:35:55Z
• Authors: Jin-zhu Wang; Ding Yuan; Xiang-hong Yang; Chang-hua Sun; Lin-lin Hou; Yan Zhang; Yan-xia Gao
• Keywords: Pathogenic bacteria; Enterococcus faecium; Acinetobacter baumannii; Klebsiella pneumonia

Introduction Pneumonia are the leading cause of death worldwide, and antibiotic treatment remains fundamental. However, conventional sputum smears or cultures still inefficient for obtaining pathogenic microorganisms.Metagenomic next-generation sequencing (mNGS) has shown great value in nucleic acid detection, however, NGS results lower respiratory tract microorganisms poorly studied. Methods This study dealt with investigating efficacy mNGS detecting pathogens patients pulmonary infections. A total 112 admitted at First Affiliated Hospital Zhengzhou University between April 30, 2018, June 2020, were enrolled this retrospective study. The bronchoalveolar lavage fluid (BALF) was obtained from each patient. Routine methods (bacterial smear culture) employed identification BALF. Results average patient age 53.0 years, 94.6% (106/112) microorganism results. detection rate significantly surpassed (93.7% vs. 32.1%, P < 0.05). Notably, 75% (84/112) found to have bacteria by mNGS, but only 28.6% (32/112) approaches. most commonly detected included Acinetobacter baumannii (19.6%), Klebsiella pneumoniae (17.9%), Pseudomonas aeruginosa (14.3%), Staphylococcus faecium (12.5%), Enterococcus Haemophilus parainfluenzae (11.6%). In 29.5% (33/112) patients, fungi identified using including 23 cases Candida albicans (20.5%), 18 Pneumocystis carinii (16.1%), 10 Aspergillus (8.9%). 7.1 % (8/112) individuals when procedures used. viruses higher than method (43.8% 0.9%, Epstein–Barr virus (15.2%), cytomegalovirus (13.4%), circovirus (8.9%), human coronavirus (4.5%), rhinovirus (4.5%). Only 29.4% positive, whereas 5.4% (6/112) negative both as Kappa analysis, indicating poor consistency two (P = 0.340; analysis). Conclusion Significant benefits been infection. For those suboptimal therapeutic responses, can provide an etiological basis, aiding precise anti-infective treatment.