Selection and validation of genes related to oxidative stress production and clearance in macrophages infected with Mycobacterium tuberculosis

• DOI: 10.3389/fcimb.2023.1324611
• Publication Date: 2023-12-12T15:31:42Z
• Authors: Renchun Su; Jinfeng Yuan; Tianhui Gao; Yuhong Liu; Wei Shu; Yufeng Wang; Yu Pang; Qi Li
• Keywords: SOD2

Background In the fight against tuberculosis, besides chemotherapy, regulation of oxidative stress (OS) has also aroused people’s interest in host-oriented therapy. However, there is limited research on genes involved reactive oxygen species (ROS) production and clearance macrophages infected with Mycobacterium tuberculosis (MTB). This study analyzes explores this to provide a basis for exploring new targets antituberculosis treatments. Methods We established macrophage model MTB, counted intracellular bacteria, determined ROS produced using flow cytometry. conducted ribonucleic acid sequencing, screened differentially expressed through transcriptomic methods, validated expression them reverse transcription-quantitative polymerase chain reaction. Results The increased bacteria at 4 h after infection MTB reached its peak 48 h, surpassing uninfected ( p < 0.05). A total 1,613 were identified which 458 associated ROS, over 50% response organelles biological processes stimuli. analyzed six genes. After CAMK2B increased, whereas CYBB decreased GPX3 SOD2 CAT Conclusion ROS-related between may be related some various processes, molecular functions, signaling pathways. Among them, CAMK2B, , ROS.