Oral infectivity through carnivorism in murine model of Trypanosoma cruzi infection

• DOI: 10.3389/fcimb.2024.1297099
• Publication Date: 2024-02-22T05:21:26Z
• Authors: Víctor Torres; Víctor Contreras; Bessy Gutiérrez; Juan San Francisco; Alejandro Catalán; José Luis Vega; Kyung-Mee Moon; Leonard J. Foster; Rafael F. de Almeida; Alexis M. Kalergis; Jorge González
• Keywords: Infectivity; Polyclonal antibodies; clone (Java method); Chagas Disease

Introduction Oral transmission of T. cruzi is probably the most frequent mechanism in wild animals. This observation led to hypothesis that consuming raw or undercooked meat from animals infected with may be responsible for transmitting infection. Therefore, general objective this study was investigate host-pathogen interactions between parasite and gastric mucosa role consumption oral cruzi. Methods Cell infectivity assays were performed on AGS cells presence absence mucin, roles pepsin acidic pH determined. Moreover, groups five female Balb/c mice fed muscle tissue obtained acute phase infection by clone H510 C8C3 hvir , monitored a parasitemia curve. Similarly, we assessed infective capacity trypomastigotes amastigotes infecting females, which orally using nasogastric probe, Finally, different trypomastigote amastigote inoculums used determine their capacities. Adhesion proteins stomach performed, adhered detected western blotting monoclonal polyclonal antibodies LC-MS/MS bioinformatics analysis. Results Trypomastigote migration mucin reduced approximately 30%, whereas at 3.5, only small proportion parasites able migrate (∼6%). ability TCTs infect 20%. In all cases, 60–100% developed high parasitemia, 80% died around day 40 post-infection. The adhesion assay showed cruzipain molecule binds cells. analysis, also confirmed transialidase, cysteine proteinases, gp63 involved attachment invasion human because they can potentially interact mucosa. addition, several mucins have protease cleavage sites. Discussion Then, under our experimental conditions, allows could when administered orally, cysteinyl proteinases trans-sialidase appear relevant molecules process.