Transcriptome and proteomic analysis of mpox virus F3L-expressing cells

• DOI: 10.3389/fcimb.2024.1354410
• Publication Date: 2024-02-13T04:29:11Z
• Authors: Yihao Wang; Junzhe Zhang; Mingzhi Li; Mengle Jia; Lingdi Yang; Ting Wang; Yu Wang; Lumei Kang; Meifeng Li; Lingbao Kong
• Keywords: KEGG; HEK 293 cells; Proteome

Background Monkeypox or mpox virus (mpox) is a double-stranded DNA that poses significant threat to global public health security. The F3 protein, encoded by mpox, an apoenzyme believed possess RNA-binding domain (dsRBD). However, limited research has been conducted on its function. In this study, we present data the transcriptomics and proteomics of F3L-transfected HEK293T cells, aiming enhance our comprehension F3L. Methods gene expression profiles pCAGGS-HA-F3L transfected cells were analyzed using RNA-seq. Proteomics was used identify study proteins interact with Real-time PCR detect mRNA levels several differentially expressed genes (DEGs) in (or Vero cells) after protein. Results A total 14,822 obtained RNA-Seq 1,672 DEGs identified, including 1,156 up-regulated 516 down-regulated genes. 27 cellular interacting identified liquid chromatography-tandem mass spectrometry (LC-MS/MS), 19 large differences abundance ratios considered be candidate proteins. Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) enrichment analyses showed significantly enriched immune-related pathways, type I interferon signaling pathway, response virus, RIG-I-like receptor NOD-like etc. Moreover, some selected further confirmed real-time results consistent transcriptome data. show are mainly related RNA splicing protein translation. Conclusions Our analysis transcriptomic proteomic (1) F3L up-regulates transcript key innate immune such as RIGI, MDA5, IRF5, IRF7, IRF9, ISG15, IFNA14 , elicits broad spectrum antiviral responses host. also increases FOS JNK while decreasing TNFR2, these factors may ultimately induce apoptosis. (2) interacts host involved translation, SNRNP70, POLR2H, HNRNPA1, DDX17, findings shed light function