Microbiome-host interactions in the pathogenesis of acute exacerbation of chronic obstructive pulmonary disease

• DOI: 10.3389/fcimb.2024.1386201
• Publication Date: 2024-07-18T04:30:47Z
• Authors: Yao Li; Xiaoyan Mao; Pengfei Shi; Zongren Wan; Dan Yang; Ting Ma; Baolan Wang; Jipeng Wang; Jingjing Wang; Rong Zhu
• Keywords: KEGG; Pathogenesis; Dysbiosis

Objective To explore the underlying mechanisms airway microbiome contributes to Acute Exacerbation of Chronic Obstructive Pulmonary Disease(AECOPD). Methods We enrolled 31 AECOPD patients and 26 stable COPD patients, their sputum samples were collected for metagenomic RNA sequencing, then subjected bioinformatic analyses. The expression host genes was validated by Quantitative Real-time PCR(qPCR) using same batch specimens. Results Our results indicated a higher Rothia mucilaginosa ( p =0.015) in group Haemophilus influenzae =0.005) group. Different expressed genes(DEGs) detected significantly enriched "type I interferon signaling pathway"( <0.001, q =0.001) gene function annotation, "Cytosolic DNA-sensing =0.002, =0.024), "Toll-like receptor =0.006, =0.045), "TNF =0.045) KEGG enrichment analysis. qPCR amplification experiment verified that OASL IL6 increased Conclusion bacteria dysbiosis may regulate pathogenesis through innate immune system pathways like type pathway Toll-like pathway.