Characterizing carbapenemase-producing Escherichia coli isolates from Spain: high genetic heterogeneity and wide geographical spread

• DOI: 10.3389/fcimb.2024.1390966
• Publication Date: 2024-05-17T07:58:05Z
• Authors: Elias Dahdouh; Laro Gómez-Marcos; Javier E. Cañada-...; Eva Ramírez de Ar...; Aida Sánchez-García; Isabel Sánchez-Ro...; Luis López-Urrutia; Pedro de la Iglesia; Alejandro Gonzale...; Jared Sotelo; Daniel Valle-Mill...; Isabela Alonso-Go...; Verónica Bautista; Noelia Lara; Silvia García-Cobos; Emilia Cercenado; Belén Aracil; Jesús Oteo-Iglesias; María Pérez-Vázquez
• Keywords: Colistin

Introduction Carbapenemase-Producing Escherichia coli (CP-Eco) isolates, though less prevalent than other CP-Enterobacterales, have the capacity to rapidly disseminate antibiotic resistance genes (ARGs) and cause serious difficult-to-treat infections. The aim of this study is phenotypically genotypically characterizing CP-Eco isolates collected from Spain better understand their mechanisms population structure. Methods Ninety representative received 2015 2020 25 provinces 59 hospitals Spanish were included. Antibiotic susceptibility was determined according EUCAST guidelines whole-genome sequencing performed. virulence-associated genes, phylogeny structure, carbapenemase genes-carrying plasmids analyzed. Results discussion 90 highly polyclonal, where most ST131, detected in 14 (15.6%) isolates. bla OXA-48 (45.6%), VIM-1 (23.3%), NDM-1 (7.8%), KPC-3 (6.7%), NDM-5 (6.7%). Forty (44.4%) resistant 6 or more groups active antibiotics colistin (98.9%), plazomicin (92.2%) cefiderocol (92.2%). Four seven cefiderocol-resistant belonged ST167 six harbored NDM . Five plazomicin-resistant rmt IncL frequent (45.7%) eight these found IncF Metallo-β-lactamases with groups, often present on same plasmid/integron. ST131 associated sat pap virulence genes. This highlights genetic versatility its potential ARGs community nosocomial