Identification of pathological-related and diagnostic potential circular RNAs in Stanford type A aortic dissection

• DOI: 10.3389/fcvm.2022.1074835
• Publication Date: 2023-01-13T04:59:44Z
• Authors: Qiao Liang; Zeyi Zhou; Hui Li; Qing Tao; Yali Wang; Anqi Lin; Jing Xu; Bin Zhang; Yongzheng Wu; Haiyan Min; Lei Wang; Shiyu Song; Dongjin Wang; Qian Gao
• Keywords: Identification

Introduction Stanford type A aortic dissection (TAAD) is one of the lethal macrovascular diseases caused by invasion blood into media layer ascending wall. Inflammation, smooth muscle dysfunction, and extracellular matrix (ECM) degradation were regarded as major pathology in affected tissue. However, expression pattern its regulation especially through circular RNAs (circRNAs) an overall characteristic TAAD molecular remain unclear. Methods We employed CIRCexplorer2 to identify circRNAs based on RNA sequencing (RNA-seq) data human tissues systematically assess role circRNA massive alterations gene aortas. The key determined LASSO model functionally annotated competing endogenous (ceRNA) network co-analysis with mRNA profile. level diagnostic capability 4 peripheral serum confirmed real-time polymerase chain reaction (RT-PCR). Results circRNAs, namely circPTGR1 (chr9:114341075-114348445[−]), circNOX4 (chr11:89069012-89106660[−]), circAMN1 (chr12:31854796-31862359[−]) circUSP3 (chr15:63845913-63855207[+]), demonstrated a high power discriminate between control tissues, suggesting that these molecules stand for difference at level. Functionally, ceRNA circRNA-miRNA-mRNA predicted online databases, combining set enrichment analysis (GSEA) cell component prediction, revealed identified covered all aspects primary pathology, centralized increasing inflammatory factors cells, ECM destruction loss vascular inherent cells along circRNAs. Importantly, we validated concentration patients. Discussion This study reinforces vital status possibility serving promising biomarkers.