Pinocembrin Decreases Atrial Fibrillation Susceptibility in a Rodent Model of Depression

• DOI: 10.3389/fcvm.2022.766477
• Publication Date: 2022-05-20T06:31:27Z
• Authors: Qian Ran; Xiaoli Chen; Cui Zhang; Weiguo Wan; Tianxin Ye; Yazhou Sun; Xin Zhao; Shaobo Shi; Bo Yang; Qingyan Zhao
• Keywords: Pinocembrin; Depression; Rodent model

Background Depression is often comorbid with cardiovascular diseases and contributes to the development maintenance of atrial fibrillation (AF). Ample research demonstrated that pinocembrin had protective effects on neuropsychiatric systems via its pharmacological properties. However, whether protects from AF in depression models not known. The present investigated antiarrhythmic underlying mechanisms depressed rats. Methods One hundred ten male Sprague Dawley rats were randomly divided into six groups: CTL group (the normal administered saline), CTP pinocembrin), MDD MDP MDA apocynin), MPA both apocynin). Chronic unpredictable mild stress (CUMS) was performed for 28 days establish model. Pinocembrin gavage Day 8 28, apocynin intraperitoneal injection 1 28. evaluated using behavioral measurements, vitro electrophysiological studies, whole-cell patch-clamp recordings, biochemical detection, Western blot, histological studies. Results treatment significantly attenuated abnormality heart rate variability (HRV), prolongation action potential duration (APD), shortening effective refractory period (ERP), reduction transient outward potassium current (I ), increase L-type calcium Ca–L which susceptibility a rat model depression. Compared rats, also increased content Kv4.2, Kv4.3, gap junction channel Cx40 decreased expression level Cav1.2, ameliorated oxidative inhibited ROS/p-p38MAPK pro-apoptotic pathway ROS/TGF-β1 pro-fibrotic pathway. Conclusion therapeutic strategy great promise patients by reducing stress.