Plasma Exosomal S1PR5 and CARNS1 as Potential Non-invasive Screening Biomarkers of Coronary Heart Disease

• DOI: 10.3389/fcvm.2022.845673
• Publication Date: 2022-06-28T05:59:18Z
• Authors: Feng Xiong; Rui Mao; Ruohan Zhao; Lijuan Zhang; Kunyue Tan; Chunxia Liu; Shuzhen Wang; Min Xu; Yi Li; Tongtong Zhang
• Keywords: 03 medical and health sciences; 0302 clinical medicine; mRNA; RC666-701; lipid metabolism; genomics; Diseases of the circulatory (Cardiovascular) system; coronary heart disease; Cardiovascular Medicine; sEVs; 3. Good health

Early diagnosis and treatment significantly improve the prognosis of coronary heart disease (CHD), but no convenient screening tools are available. This study aims to find potential non-invasive biomarkers disease.We performed microarray analysis investigate mRNA expression levels in Small extracellular vesicles (sEVs) screen differentially expressed mRNAs CHD patients vs. non-CHD patients. We then quantitative real-time polymerase chain reaction (qRT-PCR) validate results, we calculated correlations between clinicopathological data. Microarray identified 72 downregulated 31 upregulated relative patients.From study, found that sphingosine-1-phosphate receptor 5 (S1PR5) carnosine synthase 1 (CARNS1) had most significant differences patient group control group. S1PR5 was correlated with diabetes, rate, triglycerides, total cholesterol, low-density lipoprotein apolipoprotein B, fasting blood glucose (P < 0.05). CARNS1 level uric acid (UA) Overexpressed were independent risk factors for CHD. The area under receiver operating characteristic curve (AUC) 0.838 diagnosing CHD; AUC 0.883 non-CHD; plus 0.921 CHD.Microarray showed sEVs have become screening.