Notopterol Attenuates Monocrotaline-Induced Pulmonary Arterial Hypertension in Rat

• DOI: 10.3389/fcvm.2022.859422
• Publication Date: 2022-06-03T05:15:06Z
• Authors: Lin Huang; Huayang Li; Suiqing Huang; Shunjun Wang; Quan Liu; Li Luo; Shuangjiao Gan; Guangguo Fu; PeiYun Zou; Guangxian Chen; Zhongkai Wu
• Keywords: Right ventricular hypertrophy; Hypoxia

Introduction Current targeted pulmonary arterial hypertension (PAH) therapies have improved lung hemodynamics, cardiac function, and quality of life; however, none these reversed the ongoing remodeling blood vessels. Considering notopterol, a linear furocoumarin extracted from root traditional Chinese medicine Qiang-Huo ( Notopterygium incisum ), had shown antiproliferative anti-inflammatory properties in previous studies, we hypothesized that it could play role ameliorating PAH. Methods In vivo , conducted monocrotaline (MCT) induced PAH rats treated them with notopterol for 3 weeks. Then, were examined by echocardiography RV catheterization. The heart specimens harvested detection gross examination, histological examination expression inflammatory molecules. vitro human smooth muscle cells (HPASMCs) after hypoxia; then, cell proliferation was assessed counting kit-8 Edu assay, migration detected wound healing assays. Results We found mortality rate function while reducing right ventricular systolic pressure MCT-induced rats. Furthermore, reduced hypertrophy fibrosis, also eased vascular muscularization. addition, attenuated pro-inflammatory factor (IL-1β, IL-6) PCNA lungs For cultured HPASMCs subjected to hypoxia, can inhibit HPASMCs. Conclusion Our studies show exerts anti-proliferative effects arteries, which may contribute prevention