Single-Cell RNA sequencing investigation of female-male differences under PAD conditions

• DOI: 10.3389/fcvm.2023.1251141
• Publication Date: 2023-09-07T11:11:25Z
• Authors: Gloriani Sánchez ...; Nicolas Villa-Roel; Feifei Li; Christian Park; Dong-Won Kang; Katherine E. Hekman; Hanjoong Jo; Luke P. Brewster
• Keywords: peripheral arterial disease conditions; substrate stiffness; human aortic endothelial cells; RC666-701; Diseases of the circulatory (Cardiovascular) system; sex-specific differences; Cardiovascular Medicine; single-cell RNA sequencing; shear stress

Peripheral arterial disease (PAD) is an age-related medical condition affecting mostly muscular arteries of the limb. It 3rd leading cause atherosclerotic morbidity. The mechanical environment endothelial cells (ECs) in PAD characterized by disturbed blood flow (d-flow) and stiff extracellular matrices. In PAD, stiffness due to decreased elastin function increased collagen content. These parameters are largely missing from current models PAD. has been previously proven that ECs exposed d-flow or substrates lead proatherogenic pathways, but effect both, stiffness, on EC phenotype not fully investigated. this study, we sought explore sex pathways could result exposing a environment. We utilized scRNA-seq tool analyze gene expression different conditions both vitro vivo. found male stimuli presented higher genes related fibrosis vitro. validated our findings vivo murine carotid via partial artery ligation. Since women have delayed onset stiffening subsequent work may provide framework for some which biological interacts with sex-based differences