Proteomic changes to immune and inflammatory processes underlie lung preservation using ex vivo cytokine adsorption

• DOI: 10.3389/fcvm.2023.1274444
• Publication Date: 2023-10-03T04:36:18Z
• Authors: Anna Niroomand; Gabriel Hirdman; Leif Pierre; Haider Ghaidan; Sven Kjellström; Martin Stenlo; Snejana Hyllén; Franziska Olm; Sandra Lindstedt
• Keywords: Ex vivo

In recent years, the field of graft preservation has made considerable strides in improving outcomes related to solid organ restoration and regeneration. Ex vivo lung perfusion (EVLP) line with devices treatments yielded promising results within preclinical clinical studies, potential improve quality. Its main benefit is render marginal declined donor lungs suitable for transplantation, ultimately increasing pool available transplantation. addition, using such therapies machine could also increase time, facilitating logistical planning. Cytokine adsorption been demonstrated as a potentially safe effective therapy when applied EVLP circuit post-transplantation. However, mechanism by which this improves on molecular basis not yet fully understood.We hypothesized that there were characteristic inflammatory immunomodulatory differences between treated without cytokine adsorption, reflecting proteomic changes gene ontology pathways across inflammation-related proteins. study, we investigate mechanisms signaling how impacts function used during post-transplantation hemoperfusion porcine model. Lung tissues post-lung transplantation analyzed their profiles mass spectrometry.We found through set enrichment analysis immune processes coagulation significantly affected treatment after transplantation.In conclusion, showed are approach behind previously reported effects compared non-treated transplant recipients undergoing EVLP.