Open- vs. closed-chest pig models of donation after circulatory death

• DOI: 10.3389/fcvm.2024.1325160
• Publication Date: 2024-06-13T13:44:52Z
• Authors: Valentin Hubacher; Manuel Egle; Selianne Graf; Maria Arnold; Adrian Segiser; Maria Nieves Sanz; Daniela Casoni; Luisana Garcia Ca...; Kay Nettelbeck; Maks Mihalj; Matthias Siepe; Alexander Kadner; Sarah Longnus
• Keywords: warm ischemia; DCD heart transplantation; RC666-701; DCD pig model; Diseases of the circulatory (Cardiovascular) system; 610 Medicine & health; Cardiovascular Medicine; donation after circulatory death (DCD); open- vs. closed-chest physiology

BackgroundDuring donation after circulatory death (DCD), cardiac grafts are exposed to potentially damaging conditions that can impact their quality and post-transplantation outcomes. In a clinical DCD setting, patients have closed chests in most cases, while many experimental models have used open-chest conditions. We therefore aimed to investigate and characterize differences in open- vs. closed-chest porcine models.MethodsWithdrawal of life-sustaining therapy (WLST) was simulated in anesthetized juvenile male pigs by stopping mechanical ventilation following the administration of a neuromuscular block. Functional warm ischemic time (fWIT) was defined to start when systolic arterial pressure was <50 mmHg. Hemodynamic changes and blood chemistry were analyzed. Two experimental groups were compared: (i) an open-chest group with sternotomy prior to WLST and (ii) a closed-chest group with sternotomy after fWIT.ResultsHemodynamic changes during the progression from WLST to fWIT were initiated by a rapid decline in blood oxygen saturation and a subsequent cardiovascular hyperdynamic (HD) period characterized by temporary elevations in heart rates and arterial pressures in both groups. Subsequently, heart rate and systolic arterial pressure decreased until fWIT was reached. Pigs in the open-chest group displayed a more rapid transition to the HD phase after WLST, with peak heart rate and peak rate-pressure product occurring significantly earlier. Furthermore, the HD phase duration tended to be shorter and less intense (lower peak rate-pressure product) in the open-chest group than in the closed-chest group.DiscussionProgression from WLST to fWIT was more rapid, and the hemodynamic changes tended to be less pronounced in the open-chest group than in the closed-chest group. Our findings support clear differences between open- and closed-chest models of DCD. Therefore, recommendations for clinical DCD protocols based on findings in open-chest models must be interpreted with care.