Androgen Excess Induced Mitochondrial Abnormality in Ovarian Granulosa Cells in a Rat Model of Polycystic Ovary Syndrome

• DOI: 10.3389/fendo.2022.789008
• Publication Date: 2022-03-17T04:38:10Z
• Authors: Linyi Song; Jin Yu; Danying Zhang; Xi Li; Lu Chen; Zailong Cai; Chaoqin Yu
• Keywords: Follicular atresia; Androgen Excess; Granulosa cell

Androgen excess could profoundly lead to follicular dysplasia or atresia, and finally result in polycystic ovary syndrome (PCOS); however, the exact mechanism remains be fully elucidated.PCOS model rats were induced by dehydroepiandrosterone, their fertility was assessed. The ovarian granulosa cells (GCs) from matured follicles of PCOS collected identified immunofluorescence. mitochondrial ultrastructure observed transmission electron microscope function determined detecting adenosine triphosphate (ATP) content mtDNA copy number. Besides, expressions respiratory chain complexes ATP synthases relation analyzed.The successfully induced, reproductive outcomes obviously adverse. GCs layer apparently cut down distinctly destroyed. number greatly reduced, NDUFB8 ATP5j significantly down-regulated without obvious deletion 4834-bp.Androgen damage rat down-regulating expression PCOS.