Associated bacterial communities, confrontation studies, and comparative genomics reveal important interactions between Morchella with Pseudomonas spp.

• DOI: 10.3389/ffunb.2023.1285531
• Publication Date: 2023-12-13T05:48:25Z
• Authors: Guillaume Cailleau; Buck T. Hanson; Melissa Cravero; Sami Zhioua; Patrick Hilpish; Celia Ruiz; Aaron J. Robinson; Julia M. Kelliher; Demosthenes Morales; La Verne Gallegos...; Gregory Bonito; Patrick S.G. Chain; Saskia Bindschedler; Pilar Junier
• Keywords: Stenotrophomonas

Members of the fungal genus Morchella are widely known for their important ecological roles and significant economic value. In this study, we used amplicon genome sequencing to characterize bacterial communities associated with sexual fruiting bodies from wild specimens, as well vegetative mycelium sclerotia obtained isolates grown in vitro . These investigations included diverse representatives both Elata Esculenta clades. Unique community compositions were observed across various structures examined, within individual or specimens. However, specific taxa frequently detected association certain structures, providing support an core community. Bacteria Pseudomonas Ralstonia constituted associates mycelia sclerotia, while other genera (e.g., Pedobacter spp., Deviosa Bradyrhizobium spp.) bodies. Furthermore, importance a key member bacteriome was supported by isolation several strains during cultivation. Four six mycelial-derived shared 16S rDNA sequence identity sequences recovered directly examined structures. Distinct interaction phenotypes (antagonistic neutral) confrontation assays between these bacteria isolates. Genome revealed intriguing differences gene content annotated functions, specifically respect toxin-antitoxin systems, cell adhesion, chitinases, insecticidal toxins. genetic correlated phenotypes. This study provides evidence that spp. associations may greatly impact physiology.