CircARVCF Contributes to Cisplatin Resistance in Gastric Cancer by Altering miR-1205 and FGFR1

• DOI: 10.3389/fgene.2021.767590
• Publication Date: 2021-12-01T02:29:33Z
• Authors: Ruirui Zhang; Huanyu Zhao; Hongmei Yuan; Jian Wu; Haiyan Liu; Suan Sun; Zhengwei Zhang; Jiayang Wang
• Keywords: GC; resistance; 0301 basic medicine; 03 medical and health sciences; FGFR1; DDP; Genetics; miR-1205; QH426-470; circARVCF; 3. Good health

Background: Chemoresistance is a major barrier to the treatment of human cancers. Circular RNAs (circRNAs) are implicated in drug resistance cancers, including gastric cancer (GC). In this study, we aimed explore functions circRNA Armadillo Repeat gene deleted Velo-Cardio-Facial syndrome (circARVCF) cisplatin (DDP) GC. Methods: The expression circARVCF, microRNA-1205 (miR-1205) and fibroblast growth factor receptor 1 (FGFR1) was detected by quantitative real-time polymerase chain reaction (qRT-PCR), western blot assay or immunohistochemistry (IHC) assay. Cell Counting Kit-8 (CCK-8) colony formation were performed evaluate DDP cell ability. Transwell conducted assess migration invasion. Flow cytometry analysis done analyze apoptosis. Dual-luciferase reporter RNA immunoprecipitation (RIP) manipulated relationships miR-1205 FGFR1. Murine xenograft model constructed vivo. Results: CircARVCF level increased DDP-resistant GC tissues cells. silencing inhibited resistance, metastasis induced apoptosis directly interacted with inhibition reversed circARVCF silencing-mediated effect on FGFR1 served as target miR-1205. MiR-1205 overexpression restrained cells DDP, but elevation abated effect. addition, knockdown repressed Conclusion: enhanced elevating through sponging