Transcriptomic meta-analysis reveals unannotated long non-coding RNAs related to the immune response in sheep

• DOI: 10.3389/fgene.2022.1067350
• Publication Date: 2022-11-22T17:49:17Z
• Authors: Martin Bilbao-Arr...; Begoña M. Jugo
• Keywords: sheep; immune system; transcriptomics; 0303 health sciences; 03 medical and health sciences; genomics; lncRNAs; Genetics; QH426-470; RNA-seq; 3. Good health

Long non-coding RNAs (lncRNAs) are involved in several biological processes, including the immune system response to pathogens and vaccines. The annotation functional characterization of lncRNAs is more advanced humans than livestock species. Here, we take advantage increasing number high-throughput experiments deposited public databases order uniformly analyse, profile unannotated integrate 422 ovine RNA-seq samples from system. We identified 12302 lncRNA genes with support independent CAGE-seq histone modification ChIP-seq assays. Unannotated showed low expression levels sequence conservation across other mammal There were differences depending on genomic location-based classification. Differential analyses between unstimulated stimulated pathogen infection or vaccination resulted hundreds changed expression. Gene co-expression revealed gene-enriched clusters associated activation related interferon signalling, antiviral endoplasmic reticulum stress. Besides, differential networks constructed find condition-specific relationships coding lncRNAs. Overall, using a diverse set bioinformatic approaches identify an external stimulus. These findings help improvement catalogue provide sheep-specific evidence for implication general