MicroRNAs miR-142-5p, miR-150-5p, miR-320a-3p, and miR-4433b-5p in Serum and Tissue: Potential Biomarkers in Sporadic Breast Cancer

• DOI: 10.3389/fgene.2022.865472
• Publication Date: 2022-06-30T09:09:49Z
• Authors: Tamyres Mingoranc...; Guillermo Ortiz B...; Tayana Schultz Ju...; Douglas Adamoski; Rubens Silveira d...; Cleverton C. Spautz; Karina Furlan Ans...; Patricia Midori M...; Iglenir João Cavalli; Jaqueline Carvalh...; Daniela Fiori Gradia; Enilze Maria de S...
• Keywords: Extracellular vesicles

Breast cancer (BC) is a heterogeneous disease, and establishing biomarkers essential to patient management. We previously described that extracellular vesicle-derived miRNAs (EV-miRNAs) miR-142-5p, miR-150-5p, miR-320a, miR-4433b-5p in serum discriminated BC from control samples, either alone or combined panel. Using these markers, we intend evaluate whether the same markers identified EVs are also potential tissue serum. Expression analysis using RT-qPCR was performed of 67 breast patients (BC-S), 19 controls (CT), 83 fresh tumor tissues (BC-T), 29 adjacent nontumor samples (NT). In addition, The Cancer Genome Atlas (TCGA) data (832 BC-T 136 NT) performed. all comparisons, found concordant high expression levels miR-320a BC-S compared CT both cell-free (cf-miRNAs). Although miR-150-5p miR-142-5p were not be differentially expressed serum, panels including improved sensitivity specificity, supporting our previous findings EVs. Fresh TCGA database had, most an opposite behavior when EVs: lower than those NT samples. analyses revealed reduced miR-320a-3p overexpression BC-T, unlike results Brazilian cohort. showed individually could discriminate between cohort, with sensibility. Furthermore, combining higher AUC values specificity. associated poor overall survival patients. summary, observed distinguished specificity sensibility, regardless sample source. statistically significant tissue, according TCGA. When panels, combinations distinguish controls. These highlight application as biomarkers.