Comprehensive Analysis of Potential ceRNA Network and Different Degrees of Immune Cell Infiltration in Acute Respiratory Distress Syndrome

• DOI: 10.3389/fgene.2022.895629
• Publication Date: 2022-06-01T14:22:48Z
• Authors: Jiaxin Hu; Shanhui Ge; Borui Sun; Jianwei Ren; Jiang Xie; Guangfa Zhu
• Keywords: Competing Endogenous RNA; KEGG

Acute respiratory distress syndrome (ARDS) is a leading cause of death in critically ill patients due to hypoxemic failure. The specific pathogenesis underlying ARDS has not been fully elucidated. In this study, we constructed triple regulatory network involving competing endogenous RNA (ceRNA) investigate the potential mechanism and evaluated immune cell infiltration patterns patients. Overall, downloaded three microarray datasets that included 60 with sepsis-induced 79 sepsis alone from public Gene Expression Omnibus (GEO) database identified differentially expressed genes (DEGs, including 9 DElncRNAs, DEmiRNAs, 269 DEmRNAs) by R software. DEGs were subjected Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) for functional enrichment analysis, protein–protein interaction (PPI) was generated uncovering interactive relationships among DEmRNAs. Then, ceRNA contained 5 7 71 DEmRNAs established according overlapping both predicted databases. Finally, TUG1/miR-140-5p/NFE2L2 pathway as hub whole through Cytoscape. addition, distribution 22 subtypes cells recognized “Cell Type Identification Estimating Relative Subsets Known Transcripts (CIBERSORT)” algorithm, namely, naive B cells, T eosinophils. Correlations between also performed. conclusion, demonstrated new (the pathway), which may help further exploring ARDS.