Identification of prognostic biomarkers in the CMTM family genes of human ovarian cancer through bioinformatics analysis and experimental verification

• DOI: 10.3389/fgene.2022.918319
• Publication Date: 2022-08-30T06:59:31Z
• Authors: Mengjun Zhang; Jialin Wang; Haodi Yue; Lindong Zhang
• Keywords: 0301 basic medicine; 03 medical and health sciences; ovarian cancer; immune microenvironment; Genetics; biomarker; CMTM gene family; prognosis; QH426-470; 3. Good health

Background: Ovarian cancer (OV) is one of the most common gynecological malignancies worldwide, and its immunotherapy has considerable prospects. Multiple members CMTM family were aberrantly expressed in human cancers controlled key malignant biological processes immune regulation development. However, little known about function this gene ovarian cancer, especially terms immunity. Methods: GEPIA, Oncomine, HPA, Kaplan-Meier plotter, cBioPortal, GeneMANIA, TIMER used to analyze differential expression, prognostic value, genetic alterations, alterations microenvironment patients with cancer. Importantly, RT-qPCR was verify expression family. Results: CMTM1/3/4/6/7/8 showed abnormally high at mRNA protein levels OV tissues based on GEPIA HPA databases. that CMTM1/6/8 highly cell lines. IHC verified CMTM8 closely related Ki-67. Survival analysis CMTM1/2/3/5/8 can lead a significant reduction overall survival progression-free survival. There many types Also, CMTM1/2/3/6 had certain correlation changes such as infiltration checkpoint which may be potential mechanism CMTM6 PD-L1. Conclusion: This study confirmed abnormal biomarker for evaluation. target suppression checkpoints.