Cross-talk between the cytokinin, auxin, and gibberellin regulatory networks in determining parthenocarpy in cucumber

• DOI: 10.3389/fgene.2022.957360
• Publication Date: 2022-08-26T09:06:22Z
• Authors: Neha Kumari Mandal; Khushboo Kumari; Aditi Kundu; Ajay Arora; Prolay K. Bhowmick; Mir Asif Iquebal; Sarika Jaiswal; Tusar Kanti Behera; A. D. Munshi; Shyam S. Dey
• Keywords: Parthenocarpy

Cucumber is a model plant for studying parthenocarpy with abundant slicing- and pickling-type germplasm. This study was undertaken to understand the role of important cytokines (CKs), auxin (AUX) gibberellin (GA) biosynthesis degradation genes induction in slicing pickling Two genotypes gynoecious parthenocarpic cucumber, PPC-6 DG-8, along an MABC-derived non-parthenocarpic line, IMPU-1, were evaluated this study. The cucumber DG-8 strongly nature set fruit normally without pollination. Endogenous significantly higher than genotypes, whereas concentration cytokinins varied among at different developmental stages. However, exogenous application Zeatin IAA + effective inducing IMPU-1. Expression analysis CK, AUX, GA biosynthesis-related conducted PPC-6, DG-8. expression CK synthase, IPT, IPT3, PaO, LOG1, LOG2, CYP735A1, CYP735A2 up-regulated genotypes. Among transcription factor response regulators (RRs), positive regulation CSRR8/9b, CSRR8/9d, CSRR8/9e, CSRR16/17 negative feedback signalling genes, such as CsRR3/4a, CsRR3/4b, CsRR8/9a, CsRR8/9c, recorded lines. Homeostasis between cytokinin oxidases (CKXs) dehydrogenase resulted non-significant difference endogenous In addition, up-regulation key auxin-inducing proteins indicated their crucial cucumber. establishes critical CKs, regulatory networks cross-talk determining