The Single-Cell Transcriptomic Analysis of Prefrontal Pyramidal Cells and Interneurons Reveals the Neuronal Expression of Genes Encoding Antimicrobial Peptides and Immune Proteins

• DOI: 10.3389/fimmu.2021.749433
• Publication Date: 2021-10-25T13:38:01Z
• Authors: Dániel Mittli; Vanda Tukacs; András Micsonai; Lilla Ravasz; József Kardos; Gábor Juhász; Katalin Adrienna ...
• Keywords: Cell type

The investigation of the molecular background direct communication neurons and immune cells in brain is an important issue for understanding physiological pathological processes nervous system. Direct contacts between brain-infiltrating neurons, neuromodulatory effect cell-derived regulatory peptides are well established. Several aspects role glial neuro-immune also known; however, there remain many questions regarding details signaling from to cells. Thus, we report here on neuronal expression genes encoding antimicrobial immunomodulatory peptides, as proteins cell-specific activation mechanisms. In present study, analyzed single-cell sequencing data our previous transcriptomic work, obtained electrophysiologically identified pyramidal interneurons murine prefrontal cortex. We filtered out that may be associated with examined their pattern transcriptome. some these by cortical has not yet been reported. vast majority (~53%) cell protein (~94%) transcripts was transcriptome 84 cells, owing high sensitivity ultra-deep sequencing. process-related showed type-specific or enriched expression. Individual transcribed only a fraction investigated low copy numbers probably due bursting kinetics gene expression; comparison available datasets suggests functional relevance reported findings. Accordingly, propose further experimental silico studies system-related potential encoded neuroimmunological processes.