Immunoprofiling Reveals Novel Mast Cell Receptors and the Continuous Nature of Human Lung Mast Cell Heterogeneity

• DOI: 10.3389/fimmu.2021.804812
• Publication Date: 2022-01-04T07:46:54Z
• Authors: Elin Rönnberg; Daryl Zhong Hao Boey; Avinash Ravindran; Jesper Säfholm; Ann-Charlotte Orre; Mamdoh Al-Ameri; Mikael Adner; Sven-Erik Dahlén; Joakim S. Dahlin; Gunnar Nilsson
• Keywords: Chymase; Cell type

Background Immunohistochemical analysis of granule-associated proteases has revealed that human lung mast cells constitute a heterogeneous population cells, with distinct subpopulations identified. However, systematic and comprehensive cell-surface markers to study cell heterogeneity yet be performed. Methods Human were obtained from lobectomies, the expression 332 was analyzed using flow cytometry LEGENDScreen™ kit. Markers exhibited high variance selected for additional analyses reveal whether they correlated discrete discernable. Results We identified 102 surface on 23 previously not described which several showed continuous variation in their expression. Six these correlated: SUSD2, CD49a, CD326, CD34, CD66 HLA-DR. The also size granularity cells. no marker produced an profile consistent bi- or multimodal distribution. Conclusions LEGENDScreen more than 100 including that, best our knowledge, have been profiling did identify subpopulations. Instead, we demonstrate nature heterogeneity.