African swine fever virus infection activates inflammatory responses through downregulation of the anti-inflammatory molecule C1QTNF3

• DOI: 10.3389/fimmu.2022.1002616
• Publication Date: 2022-10-12T12:33:41Z
• Authors: Changjie Lv; Qiang Zhang; Li Zhao; Jingyu Yang; Zhong Zou; Ya Zhao; Chengfei Li; Xiaomei Sun; Xian Lin; Meilin Jin
• Keywords: KEGG

African swine fever (ASF) is the most dangerous pig disease, and causes enormous economic losses in global industry. However, mechanisms of ASF virus (ASFV) infection remains largely unclear. Hence, this study investigated host response to ASFV infection. We analyzed differentially expressed proteins (DEPs) between serum samples from ASFV-infected uninfected pigs using quantitative proteomics. Setting p -value < 0.05 |log 2 (fold change)| > 1.5, we identified 173 DEPs, comprising 57 upregulated 116 downregulated proteins, which belonged various biological processes pathways based on Gene Ontology Kyoto Encyclopedia Genes Genomes pathway enrichment analyses. The enriched include immune responses, metabolism, inflammation signaling pathways. Western blot analysis validated DEPs Furthermore, our proteomics data showed that C1QTNF3 regulated inflammatory pathway. knockdown led upregulation pro-inflammatory factors IL-1β, IL-8, IL-6, thus inhibiting replication. These results indicated was critical for In conclusion, revealed molecular underlying host-ASFV interaction, may contribute development novel antiviral strategies against future.