Lactate-driven macrophage polarization in the inflammatory microenvironment alleviates intestinal inflammation

• DOI: 10.3389/fimmu.2022.1013686
• Publication Date: 2022-10-18T08:03:35Z
• Authors: Hai-Cun Zhou; Wen-Wen Yu; Xin-Yan Yan; Xiao-Qin Liang; Xiu-Feng Ma; Jian-Ping Long; Xiao-Yan Du; Hong-Yan Mao; Hong-Bin Liu
• Keywords: Macrophage polarization

Lactate has long been considered an intermediate by-product of glucose metabolism. However, in recent years, accumulating evidence reveals that lactate unique biological activities. In previous studies, signaling was shown to inhibit inflammation. Furthermore, vitro experiments have can promote the transformation pro-inflammatory macrophages into anti-inflammatory macrophages. no vivo studies whether alleviate RAW 264.7 were stimulated by LPS induce M1 phenotype, and cultured with low high concentrations lactate. The cells then observed for phenotypic transformations expression inflammatory mediators surface markers. factors also analyzed cell-free supernatant fraction. Further, a mouse model DSS-induced colitis established treated Colonic tissue injury monitored histopathological examinations. showed promoted activated M2 phenotype decreased TLR4-mediated NF-κB proteins factors. model, colonic tissue, reduced inflammation organ damage, inhibited activation TLR4/NF-κB pathway, serum levels factors, increased repair intestinal mucosal barrier severity colitis. inhibits TLR/NF-κB pathway production promoting polarization addition, promotesthe protects is relatively safe. Therefore, promising effective drug treating through immunometabolism regulation.