Probiotics modulate gastrointestinal microbiota after Helicobacter pylori eradication: A multicenter randomized double-blind placebo-controlled trial

• DOI: 10.3389/fimmu.2022.1033063
• Publication Date: 2022-11-08T05:56:27Z
• Authors: Cong He; Yong Xie; Yin Zhu; Kun Zhuang; Lijuan Huo; Yong Yu; Qiang Guo; Xu Shu; Zhijuan Xiong; Zhenyu Zhang; Bin Lyu; Nonghua Lu
• Keywords: Dysbiosis

Background Helicobacter pylori ( H. ) eradication has been reported to cause short-term disruption of gut microbiota. It is acknowledged that probiotics supplementation mitigates side effects induced by eradication, yet its role on alleviating dysbiosis microbiota obscure. Objectives To evaluate the impact gastrointestinal after therapy. Methods This was a multicenter, double-blinded, randomized trial done at seven centers in China. A total 276 treatment-naïve -positive patients were randomly assigned receive 14-day bismuth-containing quadruple therapy (esomeprazole, bismuth, amoxicillin, furazolidone) combined with (Bifidobacterium Tetragenous viable Bacteria Tablets) (n=140) or placebo (n=136) for 28 days. Saliva, gastric mucosa and fecal samples collected before 16S rRNA gene sequencing. Results The incidence adverse events lower group compared (23.6% vs 37.7%, p=0.016), while there no significant difference rate. We found dramatic perturbations immediately following predominance Proteobacteria replacement commensal Firmicutes Bacteroidetes, gradually restored two weeks. reduction Bacteroidetes caused drugs neutralized supplementation. completely reconstituted depleted other taxa flourished. Of note, treated showed smaller fluctuations those placebo. also observed changes saliva illustrated overgrowth Neisseria depletion Streptococcus . expansion some pathogenic genera, including Porphyromonas , Leptotrichia mouth suppressed probiotics. Conclusion study not only demonstrated beneficial effect implementation during but provided comprehensive profile microbiome alterations along tract modulated