Plasma proteomics reveals early, broad release of chemokine, cytokine, TNF, and interferon mediators following trauma with delayed increases in a subset of chemokines and cytokines in patients that remain critically ill

• DOI: 10.3389/fimmu.2022.1038086
• Publication Date: 2022-12-01T00:09:31Z
• Authors: Jillian Bonaroti; Isabel Billiar; Hamed Moheimani; Junru Wu; Rami Namas; Shimena Li; Upendra K. Kar; Yoram Vodovotz; Matthew D. Neal; Jason L. Sperry; Timothy R. Billiar
• Keywords: Proteomics; 0303 health sciences; traumatic injury; immune mediators; Critical Illness; chemokine; Immunology; TNF; RC581-607; 3. Good health; 03 medical and health sciences; proteomics; cytokine; Humans; Cytokines; Interferons; Immunologic diseases. Allergy; Chemokines; Receptors, Cytokine

Severe injury is known to cause a systemic cytokine storm that associated with adverse outcomes. However, comprehensive assessment of the time-dependent changes in circulating levels broad spectrum protein immune mediators and soluble mediator receptors severely injured trauma patients remains uncharacterized. To address this knowledge gap, we defined temporal outcome-based patterns 184 circulation patients. Proteomics (aptamer-based assay, SomaLogic, Inc) was performed on plasma samples drawn at 0, 24, 72 hours (h) from time admission 150 patients, representative subset Prehospital Plasma during Air Medical Transport Trauma Patients Risk for Hemorrhagic Shock (PAMPer) trial. were categorized into outcome groups including Early Non-Survivors (died within h; ENS; n=38), Non-Resolvers after h or required ≥7 days intensive care; NR; n=78), Resolvers (survivors < 7 R; n=34), low Injury Severity Score (ISS) Tranexamic Acid During Hemorrhage After (STAAMP) trial as controls. The major findings include an extensive release 0h more pronounced ENS NR There selective elevated 24 greater degree multiple cytokines chemokines not previously described These validated quantitative fashion using mesoscale discovery immunoassays (MSD) external validation cohort (VC) 58 matched R status. This longitudinal description outcomes provides new level characterization response follows severe injury.