Alterations of the gut virome in patients with systemic lupus erythematosus

• DOI: 10.3389/fimmu.2022.1050895
• Publication Date: 2023-01-11T05:07:07Z
• Authors: Changming Chen; Qiulong Yan; Xueming Yao; Shenghui Li; Qingbo Lv; Guangyang Wang; Qin Zhong; Fang Tang; Zhengqi Liu; Ying Huang; Yang An; Jing Zhou; Qiongyu Zhang; Aiqin Zhang; Hayan Ullah; Yue Zhang; Can Liu; Dan Zhu; Hufan Li; Wen Sun; Wukai Ma
• Keywords: Human virome; Siphoviridae; Dysbiosis; Ruminococcus

Background Systemic lupus erythematosus (SLE) is a systemic autoimmune disease that has been linked to the dysbiosis of gut microbiome and virome. However, potential characterization virome in SLE patients needs be explored more extensively. Methods Herein, we analyzed viral community 16 31 healthy controls using both bulk virus-like particle (VLP)-based metagenomic sequencing their fecal samples. A total 15,999 non-redundant operational taxonomic units (vOTUs) were identified from assembled contigs used for profiling. Results exhibited significant decrease diversity metagenome dataset, but this change was not VLP dataset. Also, considerable alterations overall composition remarkable changes family compositions observed compared with controls, as two technologies. We 408 vOTUs (177 SLE-enriched 231 control-enriched) significantly different relative abundances between virome, 18 (17 1 The included numerous Siphoviridae , Microviridae crAss-like viruses frequently predicted infect Bacteroides Parabacteroides Ruminococcus_E while control-enriched contained members Myoviridae Prevotella Lachnospirales_CAG-274 . correlations bacteria found some Hungatella_A phages may play key roles virus-bacterium network. Furthermore, signatures discrimination achieved an area under receiver operator characteristic curve (AUC) above 0.95, suggesting prediction SLE. Conclusion Our findings demonstrated patients. Further research into etiology will open up new avenues treating preventing other diseases.