Desmosterol: A natural product derived from macroalgae modulates inflammatory response and oxidative stress pathways in intestinal epithelial cells

• DOI: 10.3389/fimmu.2022.1101643
• Publication Date: 2023-01-04T13:12:31Z
• Authors: Huan Qu; Qiufang Zong; Ping Hu; Zhaojian Li; Haifei Wang; Shenglong Wu; Hao-Yu Liu; Wenbin Bao; Demin Cai
• Keywords: 0301 basic medicine; Biological Products; Immunology; Desmosterol; algae extracts; Epithelial Cells; inflammatory response; RC581-607; Nuclear Receptor Subfamily 1, Group F, Member 3; Seaweed; desmosterol; Oxidative Stress; 03 medical and health sciences; Cholesterol; oxidative stress; Humans; Animals; Immunologic diseases. Allergy; RORγ

The serum level of cholesterol and its biosynthetic intermediates are critical indicators to access metabolism-related disorders in humans animals. However, the molecular actions these on gene functions regulation remained elusive. Here, we show that desmosterol (DES) is most abundant intermediate involved biosynthesis highly enriched red/brown algae. It exerts a pivotal role modulating core genes oxidative stress inflammatory response processes ileum epithelial cells (IPI-2I). We observed DES extracted from red algae did not affect IPI-2I cell growth or survival. A transcriptomic measurement revealed enrolled process homeostasis pathway were significantly down-regulated by treatment. Consistent with this notion, cellular reactive oxygen species (ROS) levels markedly decreased In contrast, key including IL-6 , TNF-α IFN-γ remarkably upregulated RNA-seq analysis, as further confirmed qRT-PCR. Given specific agonist nuclear receptor RORγ, also found caused elevated expression RORγ at mRNA protein levels, suggesting it potential mediator under administration. Together, results underscore vital physiological possibly via may be helpful anti-oxidation treatment immunotherapy future.