Investigations Into the Suitability of Bacterial Suspensions as Biological Indicators for Low-Energy Electron Irradiation

• DOI: 10.3389/fimmu.2022.814767
• Publication Date: 2022-04-29T05:30:34Z
• Authors: Simone Schopf; Gaby Gotzmann; Marleen Dietze; Stephanie Gerschke; Lysann Kenner; Ulla König
• Keywords: Electron beam processing

Low-energy electron irradiation is an emerging alternative technology for attenuated or complete pathogen inactivation with respect to medical, biotechnological, and pharmaceutical applications. Pathogen by ionizing radiation depends mainly on the absorbed dose. In low-energy processes, determination of dose challenging due limited, material-dependent penetration depth accelerated electrons into matter. general, there are established dosimetry systems evaluate under dry conditions. However, no system precise monitoring processes in liquids suspensions so far. Therefore, this study three different bacterial species were investigated as biological indicators, especially range low doses (< 6.5 kGy) aqueous solutions suspensions. Escherichia coli, Bacillus subtilis, Staphylococcus warneri comparatively evaluated their suitability indicators. Thin homogeneous films respective irradiated increasing electrons. The average was determined using a colorimetric dosimeter based tetrazolium salt solution. maximum minimum measured referenced film dosimeter. Subsequently, kinetics terms curves D10 values. Thus, growth assessed E. coli S. warneri. effect behavior activity bacteria studied more detail impedance spectroscopy. With delayed.