Characterization of the early cellular immune response induced by HPV vaccines

• DOI: 10.3389/fimmu.2022.863164
• Publication Date: 2022-07-18T13:06:50Z
• Authors: Hella Pasmans; Magdalena A. Berk...; Annieck M. Diks; Bas de Mooij; Rick J. Groenland; Lia de Rond; M. Alina Nicolaie; Sjoerd H. van der...; Jacques J. M. van...; Fiona R. M. van d...; Anne-Marie Buisman
• Keywords: Bivalent (engine); ELISPOT; Immunophenotyping

Introduction Current human papillomavirus (HPV) vaccines consist of virus-like particles (VLPs) which are based on the L1 protein, but they produced by different expression systems and use adjuvants. We performed in-depth immunophenotyping multiple innate adaptive immune cells after vaccination with bivalent versus nonavalent HPV vaccines. Method Twenty pre-menopausal HPV-seronegative women were enrolled randomized to receive three-doses either or vaccine. Blood samples collected at time points from baseline up 7 months first vaccination. Four extensive EuroFlow flow cytometry antibody panels used monitor various cell subsets. Additionally, HPV-specific memory B- T determined ELISPOT levels measured a VLP-based multiplex immunoassay. Results In both cohorts, numbers plasma expanded in week primary tertiary HPV16 HPV18-specific B T-cell responses higher than vaccinees one month post third For HPV31 HPV45-specific this pattern was reversed. Monocytes showed an expansion day cohorts significantly vaccine cohort. Large heterogeneity other subsets observed between donors. Conclusion This pilot study consistent response monocytes considerable variation circulating types