Gut microbiota composition reflects disease progression, severity and outcome, and dysfunctional immune responses in patients with hypertensive intracerebral hemorrhage

• DOI: 10.3389/fimmu.2022.869846
• Publication Date: 2022-10-14T08:38:05Z
• Authors: Jielian Luo; Yang Chen; Guanghai Tang; Zhuo Li; Xiaobo Yang; Xiaoxiao Shang; Tao Huang; Gan Huang; Lixin Wang; Yun Han; Yuexiang Zhou; Chuyang Wang; Bin Wu; Qihua Guo; Baoying Gong; Mengzhen Li; Ruihua Wang; Jiecong Yang; Wanzhen Cui; Jianbin Zhong; Linda Ld Zhong; Jianwen Guo
• Keywords: Prevotella

In this study, we aimed to explore the alterations in gut microbiota composition and cytokine responses related disease progression, severity, outcomes patients with hypertensive intracerebral hemorrhage (ICH).Fecal communities of 64 ICH, 46 coronary heart controls, 23 healthy controls were measured by sequencing V3-V4 region 16S ribosomal RNA (16S rRNA) gene. Serum concentrations a broad spectrum cytokines examined liquid chips ELISA. Relationships between clinical phenotypes, microbiotas, analyzed group ICH stroke-associated pneumonia (SAP), major complication ICH.In comparison control groups, had increased microbial richness diversity, an expanded facultative anaerobes opportunistic pathogens, depletion anaerobes. Enterococcus enrichment Prevotella more significant associated severity functional outcome ICH. Furthermore, also noted SAP contrast non-SAP group. Enterococci promising factors prognosis The onset induced massive, rapid activation peripheral immune system. There 12 (Eotaxin, GM-CSF, IL-8, IL-9, IL-10, IL-12p70, IL-15, IL-23, IL-1RA, IP-10, RANTES, TNF-α) changed significantly prolongation Th2 correlated 90-day outcomes. Cytokines TNF-α, IL-18, MIP-1β differed from Among these cytokines, only IP-10 levels decreased was positively IL-1RA negatively while inversely both groups.This study revealed that dysbiosis enriched depleted risk subsequently SAP. altered serum profiles are potential biomarkers reflect inciting physiologic insult/stress involved