Dietary Crude Protein Levels Alter Diarrhea Incidence, Immunity, and Intestinal Barrier Function of Huanjiang Mini-Pigs During Different Growth Stages

• DOI: 10.3389/fimmu.2022.908753
• Publication Date: 2022-07-07T09:48:26Z
• Authors: Yating Liu; Md. Abul Kalam Azad; Xichen Zhao; Qian Zhu; Xiangfeng Kong
• Keywords: Feed additive

Huanjiang mini-pig is an indigenous pig breed in China; however, the optimal dietary crude protein (CP) levels for this during different growth stages has not been standardized yet. This study investigated effects of CP on diarrhea incidence, immunity, and intestinal barrier function pigs. A total 360 mini-pigs were assigned to three independent trials fed following diets: 5−10 kg stage, 14, 16, 18, 20, 22%; 10−20 12, 20% 20−30 10, 18%. In diet increased plasma IL-1β, IL-6, IL-8, TNF-α concentrations compared 14−20% diets decreased IL-10 TGF-β; these results fluctuated later stages, including decrease IL-1β IL-8 group, 18−20% groups, increase IFN-γ group at stage 16% stage. The jejunal ileal concentration 14% as well 12% addition, was 18% Furthermore, IL-6 expression, whereas Claudin, Occludin, ZO-1, ZO-2, Mucin-1, Mucin-20 expressions TLR-2, TLR-4, NF-κB 22 respectively. Collectively, findings suggest 5−10, 10−20, respectively; provide guiding significance stages.