Gut Microflora Modulates Th17/Treg Cell Differentiation in Experimental Autoimmune Prostatitis via the Short-Chain Fatty Acid Propionate

• DOI: 10.3389/fimmu.2022.915218
• Publication Date: 2022-07-04T04:26:45Z
• Authors: He-Xi Du; Shao-Yu Yue; Di Niu; Chang Liu; Li-Gang Zhang; Jing Chen; Yang Chen; Yu Guan; Xiao-Liang Hua; Chun Li; Xian-Guo Chen; Li Zhang; Chao-Zhao Liang
• Keywords: Short-chain fatty acid; Treg cell; Chain (unit)

Chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) is a very common urological disorder and has been gradually regarded as an immune-mediated disease. Multiple studies have indicated that the gut microflora plays pivotal part in immune homeostasis autoimmune development. However, whether affects CP/CPPS, underlying mechanism behind them remain unclear. Here, we built experimental prostatitis (EAP) mouse model by subcutaneous immunity identified its Th17/Treg frequency was imbalanced. Using fecal 16s rRNA sequencing untargeted/targeted metabolomics, discovered diversity relative abundance of their metabolites were obviously different between control EAP group. Propionic acid, kind short-chain fatty acid (SCFA), decreased mice compared to controls, supplementation with propionic reduced susceptibility corrected imbalance cell differentiation vivo vitro . Furthermore, SCFA receptor G-protein-coupled 43 intracellular histone deacetylase 6 regulated Th17 Treg cells also evaluated. Lastly, observed transplantation from induced decrease recipient mice. Our data showed dysbiosis contributed via metabolite provided valuable immunological groundwork for further intervention immunologic derangement CP/CPPS targeting acid.