Tas2R signaling enhances mouse neutrophil migration via a ROCK-dependent pathway

• DOI: 10.3389/fimmu.2022.973880
• Publication Date: 2022-08-18T06:49:52Z
• Authors: Daichi Kobayashi; Tomoya Watarai; Madoka Ozawa; Yasuhiro Kanda; Fumihiro Saika; Norikazu Kiguchi; Arata Takeuchi; Masahito Ikawa; Shinsuke Matsuzaki; Tomoya Katakai
• Keywords: CXCL2; Formyl peptide receptor

Type-2 bitter taste receptors (Tas2Rs) are a large family of G protein-coupled that expressed in the oral cavity and serve to detect substances with tastes foods medicines. Recent evidence suggests Tas2Rs also extraorally, including immune cells. However, role cells remains controversial. Here, we demonstrate Tas2R126, Tas2R135, Tas2R143 mouse neutrophils, but not other such as macrophages or T B lymphocytes. Treatment bone marrow-derived neutrophils from wild-type mice Tas2R126/143 agonists arbutin d -salicin led enhanced C-X-C motif chemokine ligand 2 (CXCL2)-stimulated migration vitro , this response was observed Tas2r126/135/143 -deficient mice. Enhancement CXCL2-stimulated by Tas2R accompanied increased phosphorylation myosin light chain (MLC2) blocked pretreatment inhibitors Rho-associated coiled-coil-containing protein kinase (ROCK), small GTPase RhoA. Taken together, these results express functional suggest for Tas2R126/143–ROCK–MLC2-dependent signaling regulation neutrophil migration.