Genomics and transcriptomics reveal new molecular mechanism of vibriosis resistance in fish

• DOI: 10.3389/fimmu.2022.974604
• Publication Date: 2022-09-30T12:17:27Z
• Authors: Qian Zhou; Yadong Chen; Zhangfan Chen; Lei Wang; Xinran Ma; Jie Wang; Qihao Zhang; Songlin Chen
• Keywords: Immunology; Gene; Microbiology; selective sweep; 03 medical and health sciences; Endocrinology; Biochemistry, Genetics and Molecular Biology; Fish Immunology; Genetics; Animals; RNA-Seq; 14. Life underwater; fish disease control; Molecular Biology; Biology; Vibrio; Immunological Responses in Aquatic Organisms; Immunology and Microbiology; Dynamics and Pathogenesis of Cholera Bacteria; 0303 health sciences; Genome; FOS: Clinical medicine; Chondroitin Sulfates; Fishes; Life Sciences; Cynoglossus semilaevis; Genomics; vibriosis resistance; RC581-607; DNA Barcoding for Food Authentication and Fraud Detection; Vibrio Infections; FOS: Biological sciences; Gene expression; molecular mechanism; Immunologic diseases. Allergy; Transcriptome

Infectious diseases have caused dramatic production decline and economic loss for fish aquaculture. However, the poor understanding of disease resistance severely hampered prevention. Chinese tongue sole (Cynoglossus semilaevis) is an important flatfish suffering from vibriosis. Here we used genomic, transcriptomic experimental approaches to investigate molecular genetic mechanisms underlying vibriosis resistance. A genome-wide comparison revealed that genes under selective sweeps were enriched glycosaminoglycan (GAG) chondroitin sulfate (CS)/dermatan (DS) metabolism. Transcriptomic analyses prioritized synergic gene expression patterns in this pathway, which may lead increased CS/DS content resistant family. Further evidence showed carbohydrate sulfotransferases 12 (Chst12), a key enzyme biosynthesis, has direct antibacterial activity. To best our knowledge, first report chst12 bactericidal effect. In addition, major component extracellular matrix (ECM) selection signatures fine-tuned expressions ECM-receptor interaction indicated modification ECM structure with enhancement barrier function. Furthermore, functional studies conducted on Col6a2, encoding collagen constitutes ECM, pointed it act as cellular receptor Vibrio pathogens, thus plays role invasion. Taken together, these findings provide new insights into protective mechanism fish, offers crucial genomic resources germplasm breeding infectious control culturing.