Vascular inflammation on a chip: A scalable platform for trans-endothelial electrical resistance and immune cell migration

• DOI: 10.3389/fimmu.2023.1118624
• Publication Date: 2023-01-24T08:14:59Z
• Authors: Haley Ehlers; Arnaud Nicolas; Frederik Schavemaker; Jeroen P. M. Heij...; Martin Bulst; Sebastiaan J. Tri...; Lenie J. van den ...
• Keywords: Vascular permeability; Proinflammatory cytokine

The vasculature system plays a critical role in inflammation processes the body. Vascular inflammatory mechanisms are characterized by disruption of blood vessel wall permeability together with increased immune cell recruitment and migration. There is need to develop models that fully recapitulate changes vascular barrier response conditions. We developed scalable platform for parallel measurements trans epithelial electrical resistance (TEER) 64 perfused microfluidic HUVEC tubules under Over 250 where exposed Tumor necrosis factor alpha (TNFα) interferon gamma (INF-γ) or human peripheral mononuclear cells. was quantified based on TEER expression ICAM VE-cadherin. observed function presence both cytokines cells, decreased values, increase as well endothelial morphology. OrganoPlate 3-lane64 provide valuable tool studies an automation compatible manner. Continuous enable long term, sensitive assays studies. propose use our powerful modelling combination interaction can be used screen targets drugs treat chronic inflammation.