Tertiary lymphoid structures predict the prognosis and immunotherapy response of cholangiocarcinoma

• DOI: 10.3389/fimmu.2023.1166497
• Publication Date: 2023-05-10T17:33:04Z
• Authors: Taiyu Shang; Tianyi Jiang; Tao Lu; Hui Wang; Xiaowen Cui; Yufei Pan; Mengyou Xu; Mengmiao Pei; Zhiwen Ding; Xiaofan Feng; Yunkai Lin; Xin Li; Yexiong Tan; Feiling Feng; Hui Dong; Hongyang Wang; Liwei Dong
• Keywords: Gene signature; Clinical Significance

Introduction Cholangiocarcinoma (CCA) is a malignant tumor of the biliary epithelium with poor prognosis. The lack biomarkers to predict therapeutic response and prognosis one major challenges for CCA treatment. Tertiary lymphoid structures (TLS) provide local pivotal microenvironment immune responses. prognostic value clinical relevance TLS in remain unclear. We aimed explore characteristics significance CCA. Methods investigated using surgery cohort containing 471 patients (cohort 1) an immunotherapy 100 2). Hematoxylin eosin (H&E) immunohistochemical (IHC) staining were used evaluate maturity TLS. Multiplex IHC (mIHC) was employed characterize composition Results Different observed tissue sections. Strong four-gene signature including PAX5, TCL1A, TNFRSF13C, CD79A found regions. A high density intra-tumoral (T-score high) significantly correlated longer overall survival (OS) both 1 (p = 0.002) 2 0.01), whereas peri-tumoral (P-score associated shorter OS these two cohorts 0.003 p 0.03, respectively). Conclusion established efficiently identified tissues. abundance spatial distribution checkpoint inhibitors (ICIs) patients. presence are positive factors CCA, which theoretical basis future diagnosis treatment